LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2165..2261) /label=attL2 /note="attL2 (c)" misc_signal complement(2276..2295) /note="T7 Promoter/priming site (c)" primer_bind 2303..2319 /note="M13 Reverse priming site" gene 2432..3241 /note="Kanamycin resistance gene" rep_origin 3362..4035 /note="pUC origin" PCR_frame 669..2165 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2185) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2182) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2173..4038,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2143..2163 /note="TEV site" source 673..2142 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:156187 IMAGE:40117598" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_282" /note="Vector: pCR-Blunt II-TOPO; Clone identification sequence tag: TATACTAT sequenced from the reverse primer" gene 673..2142 /gene="UGT1A1" /gene_synonym=HUG-BR1 /gene_synonym=UDPGT /gene_synonym=UGT1*1 /gene_synonym=UGT1A /db_xref="GeneID:54658" /db_xref="HGNC:12530" /db_xref="MIM:191740" CDS 673..2142 /dnas_title="UDP glucuronosyltransferase 1 family, polypeptide A1" /gene="UGT1A1" /gene_synonym=HUG-BR1 /gene_synonym=UDPGT /gene_synonym=UGT1*1 /gene_synonym=UGT1A /codon_start=1 /product="UDP glucuronosyltransferase 1 family, polypeptide A1" /protein_id="AAI28415.1" /db_xref="GI:118763983" /db_xref="GeneID:54658" /db_xref="HGNC:12530" /db_xref="MIM:191740" /translation="MAVESQGGRPLVLGLLLCVLGPVVSHAGKILLIPVDGSHWLSML GAIQQLQQRGHEIVVLAPDASLYIRDGAFYTLKTYPVPFQREDVKESFVSLGHNVFEN DSFLQRVIKTYKKIKKDSAMLLSGCSHLLHNKELMASLAESSFDVMLTDPFLPCSPIV AQYLSLPTVFFLHALPCSLEFEATQCPNPFSYVPRPLSSHSDHMTFLQRVKNMLIAFS QNFLCDVVYSPYATLASEFLQREVTVQDLLSSASVWLFRSDFVKDYPRPIMPNMVFVG GINCLHQNPLSQEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVL WRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMP LFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDR PVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFK CCAYGYRKCLGKKGRVKKAHKSKTH" misc_feature 673..2142 /note="UGT1A1 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggctgt ggagtcccag ggcggacgcc cacttgtcct gggcctgctg 721 ctgtgtgtgc tgggcccagt ggtgtcccat gctgggaaga tactgttgat cccagtggat 781 ggcagccact ggctgagcat gcttggggcc atccagcagc tgcagcagag gggacatgaa 841 atagttgtcc tagcacctga cgcctcgttg tacatcagag acggagcatt ttacaccttg 901 aagacgtacc ctgtgccatt ccaaagggag gatgtgaaag agtcttttgt tagtctcggg 961 cataatgttt ttgagaatga ttctttcctg cagcgtgtga tcaaaacata caagaaaata 1021 aaaaaggact ctgctatgct tttgtctggc tgttcccact tactgcacaa caaggagctc 1081 atggcctccc tggcagaaag cagctttgat gtcatgctga cggacccttt ccttccttgc 1141 agccccatcg tggcccagta cctgtctctg cccactgtat tcttcttgca tgcactgcca 1201 tgcagcctgg aatttgaggc tacccagtgc cccaacccat tctcctacgt gcccaggcct 1261 ctctcctctc attcagatca catgaccttc ctgcagcggg tgaagaacat gctcattgcc 1321 ttttcacaga actttctgtg cgacgtggtt tattccccgt atgcaaccct tgcctcagaa 1381 ttccttcaga gagaggtgac tgtccaggac ctattgagct ctgcatctgt ctggctgttt 1441 agaagtgact ttgtgaagga ttaccctagg cccatcatgc ccaatatggt ttttgttggt 1501 ggaatcaact gccttcacca aaatccacta tcccaggaat ttgaagccta cattaatgct 1561 tctggagaac atggaattgt ggttttctct ttgggatcaa tggtctcaga aattccagag 1621 aagaaagcta tggcaattgc tgatgctttg ggcaaaatcc ctcagacagt cctgtggcgg 1681 tacactggaa cccgaccatc gaatcttgcg aacaacacga tacttgttaa gtggctaccc 1741 caaaacgatc tgcttggtca cccgatgacc cgtgccttta tcacccatgc tggttcccat 1801 ggtgtttatg aaagcatatg caatggcgtt cccatggtga tgatgccctt gtttggtgat 1861 cagatggaca atgcaaagcg catggagact aagggagctg gagtgaccct gaatgttctg 1921 gaaatgactt ctgaagattt agaaaatgct ctaaaagcag tcatcaatga caaaagttac 1981 aaggagaaca tcatgcgcct ctccagcctt cacaaggacc gcccggtgga gccgctggac 2041 ctggccgtgt tctgggtgga gtttgtgatg aggcacaagg gcgcgccaca cctgcgcccc 2101 gcagcccacg acctcacctg gtaccagtac cattccttgg acgaaaactt gtactttcaa 2161 ggctCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG 2221 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC 2281 CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA 2341 AAATCTCTGA TGTTACATTG CACAAGATAA AATAATATCA TCATGAACAA TAAAACTGTC 2401 TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG 2461 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA 2521 TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT 2581 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT 2641 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA 2701 TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA 2761 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA 2821 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC 2881 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG 2941 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC 3001 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT 3061 AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT 3121 ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG 3181 TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA 3241 ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG 3301 CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT GAGCGTCAGA 3361 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 3421 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 3481 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT 3541 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 3601 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 3661 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 3721 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 3781 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 3841 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 3901 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 3961 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 4021 GCCTTTTGCT CACATGTT //